core laboratories

The UNCC Microarray Core Facility is a joint project by UNCC and Carolinas Medical Center researchers and primarily funded by NC Biotech. The facility is equipped for small-batch printing of oligonucleotide and cDNA glass-slide microarrays. The facility grant was awarded in August of 2006. The facility, located in 273 CARC, is supervised by Dr. Cynthia Gibas and staffed by a full-time technician, and will be accessible to all UNCC researchers on a cost-recovery basis beginning in spring 2007.

Major equipment:

  • Matrix PlateMate 2x2 robotic system for automated microvolume pipetting, including plate replication and reformatting, reagent addition, serial dilution, and reaction setup;
  • BioRad BioOdyssey Calligrapher benchtop microarray fabrication system for printing of small- to medium- batches of microarrays on standard slides, on membranes or in microplates;
  • Tecan HS-4800 automated hybridization and wash station for processing of multiple microarrays under controlled conditions;
  • Tecan LS-Reloaded microarray scanner with capabilities for multi-slide and microplate scanning and up to three dye chemistries simultaneously in red, green and blue wavelength ranges;

Other equipment in the core facility includes a Bio-Rad iQ5 Real Time PCR system with 5-color detection capabilities, an Agilent eBioanalyzer for QC of nucleic acid and protein samples, a NanoDrop UV-Vis spectrophotometer for quantitation of nucleic acids in extremely small sample volumes, and ThermoElectron and Agilent hybridization ovens, carousels, and chambers for manual hybridization of glass-slide microarrays.

Staff: Mr. Joshua Newton, 273A Cameron Applied Research Center