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faculty profile |
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Research Description:
The major objective of my research is to understand the mechanisms of viral RNA replication and transcription in the nonsegmented negative-strand RNA viruses (the order Mononegavirales) represented by a wide variety of human, animal (vertebrates as well as invertebrates) and plant viruses, where the RNA polymerase complex possesses unique features. The order Mononegavirales includes many important human and animal pathogens, such as rabies, measles, Ebola, Marburg, Nipah, Hendra, Borna disease, parainfluenza, Newcastle disease, rinderpest, and other viruses. Importantly, the Ebola and Marburg viruses are classified as category A bioweapon agents and are currently considered to be one of the bioterrorism threats. A detailed understanding of the mode RNA synthesis in these viruses is fundamental to developing reagents to prevent and combat these deadly diseases. To provide insights into the biology of these viruses, my studies employ two prototypic Mononegavirales, vesicular stomatitis virus (VSV, a rhabdovirus) and Sendai virus (paramyxovirus), which serve as attractive models due to a lack of serious pathogenicity for humans and their very efficient replication in a wide range of cell lines. The two-virus approach allows the identification of universal as well as virus-specific mechanisms of viral RNA replication/transcription, because VSV and Sendai virus belong to the different families within the order Mononegavirales. Another direction of my research will focus on the exploitation of VSV and Sendai virus as clinically important vaccine and oncolytic vectors. |
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Recent Publications:
Furr S.R., Chauhan V.S., Sterka D.Jr., Grdzelishvili V., and Marriott I. 2008 “Characterization of retinoic acid-inducible gene-I expression in primary murine glia following exposure to vesicular stomatitis virus”, Journal of Neurovirology, in press.
Grdzelishvili V.Z., Smallwood S., Tower D., Hall R.L., Hunt D.M., and Moyer S.A. 2006 “Identification of a new region in the vesicular stomatitis virus L protein which determines defects in mRNA cap methylation”, Virology 350, 394-405
Grdzelishvili V.Z., Smallwood S., Tower D., Hall R.L., Hunt D.M., and Moyer S.A. 2005 “A single amino acid change in the L polymerase protein of vesicular stomatitis virus completely abolishes viral mRNA cap methylation”, Journal of Virology 79, 7327-7337
Grdzelishvili V.Z., Garcia-Ruiz H., Watanabe T., and Ahlquist P. 2005 “Mutual interference between genomic RNA replication and subgenomic RNA transcription in brome mosaic virus”, Journal of Virology 79, 1438-1451
Kushner D.B, Lindenbach B.D., Grdzelishvili V.Z., Noueiry A.O., Paul S.M., and Ahlquist P. 2003 “Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus”, Proc. Natl. Acad. Sci. USA 100, 15764-15769
Grdzelishvili V.Z., Chapman S.N., Dawson W.O. and Lewandowski D.J. 2000 “Mapping of the tobacco mosaic virus movement protein and coat protein subgenomic RNA promoters in vivo”, Virology 275, 177-192
Solovyev, A. G., Savenkov E. I., Grdzelishvili V. Z., Kalinina N. O., Morozov S. Y., Schiemann J., and Atabekov J. G. 1999 “Movement of hordeivirus hybrids with exchanges in the triple gene block”, Virology 253, 278-287
Solovyev, A. G., Zelenina D. A., Savenkov E. I., Grdzelishvili V. Z., Morozov S. Y., Maiss E., Casper R., and Atabekov J. G.. 1997 “Host-controlled cell-to-cell movement of a hybrid barley stripe mosaic virus expressing a dianthovirus movement protein”, Intervirology 40,1-6
Solovyev, A. G., Zelenina D. A., Savenkov E. I., Grdzelishvili V. Z., Morozov S. Y., Lesemann D. E., Maiss E., Casper R., and Atabekov J. G.. 1996 “Movement of a barley stripe mosaic virus chimera with a tobacco mosaic virus movement protein”, Virology 217, 435-441.
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