UNC Charlotte and the Carolinas Medical Center have collaborated to bring laboratory resources for high-throughput molecular biology research to the Charlotte Area. Available facilities on the CMC campus include mass spectroscopy and Affymetrix microarray processing.
Mass Spectrometer Facility
The Mass Spectrometer Facility is a joint project by UNC-Charlotte and Carolinas Medical Center researchers and primarily funded by NC Biotech. The facility was awarded in May 2006. The facility is located at Molecular core laboratory in Cannon Research Center at CMC, supervised by Dr. M. Taghi Mostafavi and managed by the core facility manager and a full-time mass spectrometer specialist (starting in Spring 2007). In addition a part time IT staff and a molecular biology technician will support researchers from both institutes for use of this facility.
Major equipment:
LTQXL Linear Quadruple Ion Trap Mass Spectrometer bundle that include high performance 2-D Linear Ion Trap Mass Spectrometer with Xcalibur and Bioworks 3.3 Software;
NLC-2D-20-1 Nano LC 2-dimensional binary gradient pump system with external 10-port column switching valve, configured for 20 ul/min max flow in first dimension and 1 uL/ min max;
NLC-AS1 AS-1 temperature controlled autosampler with 96 well capacity that includes six port injection valve flow in second dimension;
New Objective NanoSpray Probe with PicoView 550 for LTQXL Ion Trap Mass spectrometer;
Data are accessible by all researchers at UNC-Charlotte and CMC through network connected to a server with Bioworks software.
Staff: TBD
Affymetrix Microarray Facility
The Affymetrix Microarray Facility is joint project by UNC-Charlotte and Carolina Medical Center researchers located in the Molecular Biology Core Facilities in Cannon Research Center, CMC. This facility is managed by Dr. Nury Steuerwald at UNC-Charlotte and the manager of the core facility, Z. Bahrani-Mostafavi, at CMC.
Major equipment:
Genechip Fluidics Station 400;
Genechip Hybridization Oven 640;
Affymetrix Genechip Scanner 3000;
The facility includes the latest software by affymetrix for data analysis and genomics study. The core facility laboratory is staffed by technicians who will prepare samples and provide data from the microarray system to researchers at both institutions.
